Overview on the role of dietary Spirulina platensis on immune responses against Edwardsiellosis among Oreochromis niloticus fish farms

Edwardsiellosis is a bacterial fish disease that mostly occurs in freshwater farms and is characterized by a high mortality rate. Edwardsiella tarda strain was recovered from 17 fish out of 50 Nile tilapia, which were harboring clinical signs of systemic septicemia. The level of un-ionized ammonia (NH3) in the fish farm’s water was 0.11–0.15 mg/L, which was stressful for the Nile tilapia. Sequencing of the gyrB1 gene confirmed that the isolate was E. tarda JALO4, and it was submitted to NCBI under the accession number PP449014. The isolated E. tarda harbored the virulence gene edw1 AHL-synthase (quorum sensing). In addition, the isolate was sensitive to trimethoprim and sulfamethoxazole mean while it was intermediate to florfenicol. The median lethal dose (LD50) of E. tarda JALO4 was determined to be 1.7 × 105 CFU/mL in Nile tilapia. In the indoor experiment, Nile tilapia (45.05 ± 0.4 g), which received dietary Spirulina platensis (5 and 10 g/kg fish feed), showed optimum growth and feed utilization. Meanwhile, after receiving dietary S. platensis, the fish’s feed conversion ratio (FCR) was significantly enhanced compared to the control, which was 1.94, 1.99, and 2.88, respectively. The expression of immune-related genes interleukin (IL)-1β and tumor necrosis factor (TNF)-α were upsurged in E. tarda-challenged fish with higher intensity in S. platensis groups. Dietary S. platensis at a dose of 10 g/kg fish feed could provide a relative protection level (RPL) of 22.2% Nile tilapia challenged against E. tarda. Nile tilapia experimentally infected E. tarda, drastically altering their behavior: higher operculum movement, low food apprehension, and abnormal swimming dietary S. platensis (10 g/kg fish feed) could rapidly restore normal status. It was concluded that Edwardsiellosis could alter Nile tilapia behavior with a high loss in fish population. Fish received dietary-S. platensis could rapidly restore normal behavior after E. tarda infection. It is recommended the incorporation of S. platensis at doses of 10 g/kg into the Nile tilapia diet to boost their immunity and counteract E. tarda infection. Supplementary Information The online version contains supplementary material available at 10.1186/s12917-024-04131-7.


Introduction
Aquatic animals are poikilothermic, and their immune responses are affected by the surrounding environment, such as water parameters salinity, temperature, pH, dissolved oxygen, and ammonia compounds [1,2].Nile tilapia (Oreochromis niloticus) belongs to the Cichlidae family that originated from Africa, and it can tolerate a wide range of water temperature16-38 °C; beyond this level, health hazards occurred due to deterioration of several metabolic functions resulting in a decrease of feed intake and immune suppression becoming more vulnerable to opportunistic pathogens, even become lethal below 10 °C [3,4].
Microbial diseases are a major constraint in aquaculture and cause annual fish losses, job casualties, economic crises, and food insecurity; fish pathogens are mainly opportunistic, affecting immune-depressed fish [5][6][7][8].The genus Edwardsiella belongs to the Enterobacteriaceae family had five species that are pathogenic to fish: E. tarda, E. ictaluri, E. hoshinae, E. piscicida according to Abayneh et al. [9], and E. anguillarum according to Shao et al. [10], E. tarda is a Gram-negative bacilli that is known as highly pathogenic bacteria in both humans and aquatic animals [11,12].Several studies have shown phenotypic variations and interspecific diversity between isolates retrieved from different geographic regions and host species [13].Therefore, rapid and precise detection of the causative agent is a principal issue for the control and epidemiological studies [14].It affects many aquatic species, causing mass mortality and considerable economic losses worldwide [15].Several reports on the occurrence of Edwardsiellosis in non-catfish species included the Rosy Barb (Puntius conchonius) [16], Nile tilapia in the Western Hemisphere [17], and zebrafish (Danio rerio) [18].
Circumstances leading to immune suppression are inevitable in aquaculture (open system), so natural immune promoters could manage Nile tilapia to withstand opportunistic pathogens.
Spirulina platensis is a microscopic, filamentous, multicellular, and photosynthetic blue-green alga commonly used as a safe and functional supplement in animal feed.S. platensis possesses a smooth body with a delicate cell wall, making it highly digestible [19,20].S. platensis is a nutritionally dense cyanobacteria containing appreciable levels of amino acids, fatty acids, vitamins, minerals, pigments, and digestive enzymes that are considered beneficial to health and improve fish resistance against pathogens and environmental stressors [21][22][23].S. platensis has two distinctive antioxidant pigments (carotenoids and phycocyanin) and vitamins (E and C), in addition to chlorophyll, fucoxanthin, polyphenols, and polysaccharides [23,24].S. platensis enhanced nonspecific immunity of channel catfish (Ictalurus punctatus) and increased host defense mechanisms [25], Nile tilapia [26].Meanwhile, Watanuki et al. [27] found that dietary S. platensis could stimulate the immune system of common carp (Cyprirus carpio).
In this work, we investigate the bacterial pathogen that was accompanied by high mortality in freshwater fish farms, the alteration in fish behavior and immune responses associated with bacterial infection, and the possible amelioration role of S. platensis.

Case history and sampling
Nile tilapia (Oreochromis niloticus) weighing 80-120 gm were cultured in earthen ponds at a density of 30,000 per acre; paddlewheel aerators were not used on the farm.Fish experienced approximately 50 deaths/acre/ day during the early summer of 2023 on a private farm in Kafrelsheikh governorate, Egypt.Moribund fish had low feed intake, anorexia, abnormal swimming behavior, lethargy, skin ulcerations, and hemorrhage.
Moribund and dead Nile tilapia from the affected fish farm were transferred to the bacteriology laboratory of fish diseases at the Animal Health Research Institute (AHRI) within 1-2 h using an ice box.The fish were examined for clinical signs and post-mortem lesions of fish diseases [28].

Examination of fish farm water
On fish farm, physical water parameters were determined including water temperature and salinity (model YSI environmental, EC300), dissolved oxygen (DO) (Aqualytic, OX24), and pH (Thermo Orion, model 420 A).
Samples of farm water were collected in septic polyethylene bottles to determine the concentration of ammonia compounds including: total ammonia nitrogen (TAN), unionized ammonia (NH 3 ), nitrite (NO 2 ), and nitrate (NO 3 ), samples were transferred on ice to the laboratory of Animal Health Research Institute (AHRI), and were analyzed using a UV/Visible spectrophotometer, Thermo-Spectronic 300 [29].

Bacteriological examination Isolation, phenotypic and biochemical identification of Edwardsiella spp
The preliminary identification of bacterial isolates: Swabs were taken from the Nile tilapia organs, kidney, heart, liver, and spleen, then cultivated into tryptic soy broth (TSB) at 30 °C/24 h.Loopfuls from the bacterial broth culture were cultivated onto agar tryptic soy with 5% sheep blood and incubated at 30 °C/24 h.Prevailed colonies were randomly selected and purified by sub-culturing onto TSA.The isolated colonies were sub-cultivated onto selective agar Salmonella-Shigella agar.Bacterial isolates were stored at -80 °C in tryptic soy broth with glycerol for further analysis.The isolate was identified using the Gram-stain and API 20E (bio-Merieux) [30,31].All bacterial media are manufactured by Difco, Detroit, USA.

DNA extraction and polymerase chain reaction PCR
To identify the bacterial isolate, the bacterial DNA was extracted and examined for the presence of the E. tarda gyrB1 gene.It was also tested for virulencedeterminant genes, including Cds1 chondroitinase, edwI AHL-synthase, qseC sensor protein, and pvsA vibrioferrin synthesis.The extraction process of bacterial DNA was performed using the PathoGene-spin™ DNA Extraction Kit (iNtRON Biotechnology, Seongnam, Korea).Briefly, the PCR reaction consisted of a 25 µl mix containing 12.5 µl of 2X MyTaq HS mix (Bioline, Meridian Life Science, UK); 1 µL of each primer, 100 ng of template DNA; and RNase-free water.The PCR products were separated using electrophoresis on agarose gel 1% (Applichem, Germany, GmbH) and were photographed by a gel documentation system (Alpha Innotech, Biometra).The primers and PCR conditions for gene amplification are in Table 1.

Sequencing for strain identification
The extracted DNA was adjusted to 50 ng/µl and then stored at − 20 ˚C for sequencing.The isolate of Edwardsiella strain was identified and discriminated based on DNA sequencing analysis of E. tarda gyrB1.The amplicons were excised from the gel and purified using the QIAquick extraction kit (Qiagen, Germany).Nucleotide sequencing of the amplified genes was performed using the Big Dye terminator v3.1 kit (Life Technologies, Applied Biosystems, Foster City, CA, USA) by ABI 3730xl DNA sequencer (Applied Biosystems™, USA) using a primer of the PCR reaction in both directions.The raw sequence was manually checked and edited for ambiguities using a sequence alignment editor (BioEdit v. 7.2.5)[32].Finally, the edited sequence was submitted to the GenBank database to get the accession number.

Phylogenetic tree
The Neighbor-Joining phylogenetic tree was conducted to match the gyrB1 of Edwardsiella spp. with the accession numbers of typing strains retrieved from the GenBank database using MEGA X [33].The phylogenetic tree of sodB gene was rooted on Vibrio anguillarum strain ATCC 43,314 (KU755359.1),which was used as an outgroup.The lineage supports were assessed using nonparametric bootstrapping with 1000 replicates.The following factors were also employed: substitutions: transversions and transitions; pattern among lineages: homogeneous; and 95% cutoff partial deletion principal.

Gene expression of immune-related genes
The impact of dietary S. platensis on the expression of pro-inflammatory genes in the head kidney of the experimental Nile tilapia.The interleukin (IL)-1β and tumor necrosis factor (TNF)-α genes (Table 1) was assessed using quantitative real-time polymerase chain reaction (RT-PCR).The RNA was extracted with Trizol reagent (iNtRON Biotechnology Inc., Korea), and samples were collected from three Nile tilapia, each group using Nanodrop D-1000 spectrophotometer (NanoDrop Technologies Inc., USA).The obtained RNA was assessed for quality and quantity and kept at − 80 °C.The β-actin was used as a housekeeping gene.The results were assessed using Eq. 2 −ΔΔCT [34].

Lethal median dose and pathogenicity testing
The LD 50 was determined briefly according to the procedure described by Reed and Muench [37], briefly.After acclimatization at the wet laboratory of the

Feeding trial
In an indoor trial to improve the performance of Nile tilapia to combat the recovered pathogens, the green algae Spirulina platensis was added to the fish diet.Nile tilapia was collected from freshwater fish farm with no history of high mortality or health troubles.Fish weighing 45.05 ± 0.4 g was tranquilized in the fish farm and then swiftly transferred in labeled plastic bags filled with 1/3 clean water and 2/3 pure oxygen.At the farm site, fish were tranquilized using anesthetic agent MS-222 (tricaine methanesulfonate) at a dose of 40 mg/L of transporting water; MS-222 is produced by Syndel, Canada.Fish were subjected to an iodine bath at 20 ppm/10 minutes (Betadine®, the active ingredient, is 5% of povidoneiodine and produced by the Nile Company for Pharmaceuticals) at the arrival in the wet laboratory at Animal Health Research Institute, Agriculture Research Center, Kafrelsheikh Egypt [40,41].
With meticulous precision, the fish food (in pellet form) was soaked and fully homogenated until a paste was formed.The gelatin (Canal AquaCure, Egypt) was then added to the feed paste/S.platensis mixture, enhancing the feed's consistency.This mixture was left to dry at room temperature and subsequently cut into small, uniform sizes.
The feeding trial lasted for four weeks prior to the experimental infection.Fish feed was offered at 0.9.00 a.m. and 03.30 p.m. at a rate of 5% b.w.fish per day, with a basal diet composition of crude protein of 30.95% and digestible energy of 3400.5 kcal/kg.The diet was purchased from the local market and manufactured by Aller Aqua® Egypt (Reg.no.9782).Lot.no.365,622 https:// www.aller-aqua.com/.
The variation in the feeding rate of the fish's body weight was adjusted weekly.The growth performance and feed utilization were determined using the following equations, Where final body weight (FW), initial body weight (IW), daily weight gain (DWG) feed conversion ratio (FCR), and t = time (days).

Experimental infection
To examine the pathogenicity of isolated E. tarda, 15 fish experimental fed S. platensis and control (control positive) were injected (I/P) with 0.1 mL of a bacterial solution containing LD 50 1.7 × 10 5 CFU/mL, the bacteria dose was adjusted to LD 50 using the McFarland scale in phosphate buffer saline.At the same time, a negative control group was schooled by injecting 15 fish with pure saline 0.65 mg/l in concentration (control negative) [42].Each dead fish was counted if E. tarda was re-isolated for 14 days.the mortality rate (MR) and relative protection level RPL [43] were estimated using the following equations: MR% = number of deaths in a specific period total population during that period × 100 RLP% = (1 -%deaths in the treated group %deaths in the control group ) × 100 Bacteria were re-isolated following the scheme in the above section.The phenotypic profiles of E. tarda were done according to Bergey's recommendations [44], and biochemical characters were illustrated using API 20E strips [45].

Fish reflexes
Fish breath was assessed by operculum movement (OM), which was recorded by visually counting the number of opercular beats for 1 min at one-h intervals [46].Scores classify feeding, capture indicators, and respected characteristics for on-farm tilapia welfare assessment [47].Food apprehension was scored into four levels:

Histopathological examination
At the end of the feeding trial and bacterial challenge, the gills, liver, and spleen specimens were taken and preserved in buffered formalin (10%) for histopathological analyses.Specimens were dehydrated with alcohol and cleared with xylol.They were embedded in paraffin wax and cut into 4 μm sections.Specimens were stained with hematoxylin-eosin and examined using a light microscope [48].

Statistical analyses
Data concerning the ameliorating role of S. platensis to Edwardsiellosis in the experimental Nile tilapia were analyzed to determine the variance (ANOVA).Significance between the experimental groups using one-way ANOVA test and data were expressed as mean ± standard error (SE) at the significant difference of P ≤ 0.05 (the SPSS software for Windows, SPSS Inc., Chicago, IL, USA).
It was recorded that out of 50 Nile tilapia harboring clinical signs, 17 fish were infected with E. tarda.Clinical signs were low feed intake, external hemorrhages on the body surface and fin bases, and dentated tail fin (tail rot), whereas postmortem signs were enlarged brownish-red liver, distended gall bladder, splenomegaly, and inflamed intestine with ascetic fluids (Fig. 1).

Bacteria identification and median lethal dose LD 50
By using API 20E, 17 out of 50 Nile tilapia were positive for E. tarda, which was identified using API20 E with the identification number (4,144,000), a unique identifier that aids in the classification and tracking of this bacterium.
To determine LD 50 , Nile tilapia weighting 50 g were subjected to descending ten-fold dilutions of the isolated E. tarda JALO4.The LD 50 was found to be 1.7 × 10 5 CFU/ mL at water temperature 27.5 ± 2.5 °C, salinity 0.23 g/L, pH 7.9, and DO 5.8 ± 1.5 mg/L.
In Table 2, the isolated E. tarda was sensitive to trimethoprim 1.25 µg & sulfamethoxazole 23.75 µg and intermediate to florfenicol 30 µg, meanwhile it was resistant to tetracycline, ciprofloxacin, erythromycin, gentamycin, amoxicillin, ampicillin, kanamycin, cefotaxime, and streptomycin.These findings indicate a significant challenge in treating E. tarda infections due to its resistance to a wide range of commonly used antibiotics.The obtained MDR was 0.8, indicating that E. tarda JALO4 is a multiple antibiotic-resistant bacteria.

Growth performance
In Table 3, Nile tilapia (45.05 ± 0.4 g) were supplemented with dietary S. platensis at a dose of 5 and 10 g/kg diet.The growth parameters FW (56.87 and 57.46 g), WG (11.67 and 12.23 g), DWG (0.42 and 0.44 g), and FI (12.2 and 23.66 g) were significantly increased in fish that received dietary S. platensis compared to the control 51.2 g, 6.53 g, 0.23 g, and 18.73 g, respectively.A significant enhancement of feed utilization was achieved in S. platensis groups as FCR was 1.94 and 1.99 compared to the control 2.88.

The expression of immune-related genes
Gene expression of pro-inflammatory IL-1β (Fig. 4) and TNF-α (Fig. 5) were affected by both dietary S. platensis and E. tarda infection.Post-infection by 3 h, IL-1β expression was significantly upsurged with E. tarda infection 2.78 and 4.42 fold-change in the head kidney of Nile tilapia received S. platensis at a dose of 5 g/kg fish feed and 10 g/kg fish feed, respectively, compared to infected control positive (+) and uninfected control negative (-) 1.12 and 0.3 fold-change, respectively.Following the trend of IL-1β expression, TNF-α expression was upsurged in response to E. tarda infection in fish that   received S. platensis at doses of 5 g and 10 g/kg fish feed compared to infected control.After 48 h post-infection, the gene expression of IL-1β and TNF-α expression significantly decreased compared with 3-h post-infection.

Experimental infection and relative protection level of S. platensis
In Fig. 6, clinical signs of the experimentally infected Nile tilapia were similar to those obtained with naturally infected ones: external body discoloration (paleness), scale loss, and slight exophthalmia.Also, post-mortem were brownish hemorrhagic liver, distended gall bladder, splenomegaly, and a partially empty intestine with greenish content.
In Table 4, MR% was decreased in the fish group that received S. platensis at a dose of 10 g/kg fish feed compared with the control and those that received 5 g /kg fish feed: 46.67%, 60%, and 60%, respectively.Supplementation of Nile tilapia with S. platensis at a dose of 10 g/kg fish feed could provide a RPL of 22.2%.

Fish behavioral reflexes at 3 and 14 days post-infection
In Table 5, Nile tilapia experimentally infected with E. tarda showed drastic behavior changes; OM (opercular beats for 1 min period at 1 h interval) was increased regardless of S. platensis supplementations.Meanwhile, OM was higher in fish that received dietary S. platensis 55-61 opercular beats in the 3 days post-infection, restoring average values in 14 days compared to the control.Food apprehension was decreased in response to E. tarda infection, and S. platensis supplementation could enhance and restore average values after 14 days postinfection.Nile tilapia experimentally infected with E. tarda lost their normal swimming behavior, and dietary S. platensis could reserve normal behavior in the 3 days post-infection; 14 days post infections survived, and fish could normally swim.

Histopathological analyses pre-and post-infection
In Fig. 7, histological features of the gills of control fish (a), fish received 5 g (b) and 10 g (c) /kg fish feed S. platensis were normal.In contrast, fish of the control and infected with E. tarda (d) showed truncated lamellae and tips sloughing.Fish (e) received S. platensis and infected with E. tarda had lamellar aneurysm.In Fig. 8, fish in the control and fish feed S. platensis groups (a, b, and c) showed normal hepatic-parenchyma and development of pancreatic acini with the occurrence of some melanomacrophage cells.The hepatic tissue of control fish infected with E. tarda (d) showed liquifactive necrosis in the form of a wide area of lysed hepatic-parenchyma, fish received S. platensis and infected with E. tarda (e) hepatic had a small lysed area in the hepatic-parenchyma.In Fig. 9, control fish infected with E. tarda (d) showed marked depletion of lymphocytic elements without melano-macrophage centers, whereas some alterations were recorded in control (a), which were moderately sized melano-macrophage centers.In contrast, fish fed S. platensis (b) and (c) showed wide sized foci of melanomacrophage, dispersed and melano-macrophage cells.A moderate sized melano-macrophage centers in fish fed S. platensis and infected with E. tarda (e).In Fig. 10, Muscle bundles; normal histological criteria of muscle bundles in control fish (a) and fish received S. platensis (b).Muscle bundles of control fish infected with E. tarda showed foci of bacteria aggregation, vacuolation of (white arrows, c), Evidence of muscle regeneration in the form of increased cellularity, split bundles of fish received 5 g/kg fish feed S. platensis and infected with E. tarda (d) and corrugation in fish received 10 g/kg fish feed S. platensis and infected with E. tarda (e).

Discussion
In this work, the concentration of ammonia compounds of the affected fish farm was high enough to be stressful and immune depressed for Nile tilapia, including TAN (0.98-1.29 mg/L), NH 3 (0.11-0.15 mg/L), NO 2 (0.04-0.06 mg/L), and NO 3 (0.88-0.93 mg/L) despite partial replace of the pond water.In accordance, Nile tilapia that were exposed to 0.53 and 0.265 mg/L of NH 3 and infected with Clostridium perfringens type A suffered from high MR 80% and 70%, respectively [49], these results could be due to impairment of the immune responses that resulted in an upsurge of the vulnerability of fish to bacterial infection [50].Likely, the safe maximum concentration of NH 3 is 0.1 mg/L for short-term in freshwater fish [51] and 0.012 mg/L seawater for long-term in Atlantic salmon posts molts (Salmo salar L.) [52].Nile tilapia naturally and experimentally infected with E. tarda showed similar clinical signs and post-mortem lesions that were signs of septicemic bacterial diseases, including external hemorrhages, tail rot, ascites, splenomegaly, and inflamed intestines.Similarly, both freshwater fish O. niloticus and Clarias gariepinus naturally infected with E. tarda had skin ulcers and hemorrhages, ascites, muscular hemorrhage, congested liver with splenomegaly; these signs were similar to those observed with the experimentally infected fish [53], in addition, Rao et al. [54] and Leung et al. [55] claimed that E. tarda produced bacterial toxins (various virulence factors) such as hemolysin, catalase and extracellular products (ECPs) that could attack the host cells causing tissue In the last decades, bacterial identification has relied on genetic-based investigations to avoid the shortcomings of conventional methods [56,57].In this work, naturally diseased Nile tilapia were positive for E. tarda, PCR, and sequence of gyrB1.Accordingly, Lan et al. [56] found that the gyrB1 gene could be used as a marker to screen the presence, identification, and classification of Edwardseilla species, while the edw1 gene was used to confirm the virulence and pathogenicity of E. tarda [58].Similarly, in Egypt, isolated E. tarda harbored edwI and qseC genes [53], and pvsA gene and edw1 gene [59].These genes (edwI, and qseC genes) are responsible for the virulence of E. tarda by regulating flagellar motility, biofilm formation, and quorum sensing [60].
In the indoor bacterial challenge test, we determined the LD 50 of E. tarda to be 1.7 × 10 5 CFU/mL for Nile tilapia (50 g) under specific water conditions.It is important to note that LD 50 can vary significantly among different fish species.For instance, different LD 50 values were detected for freshwater fish [61], African catfish and Nile Tilapia [62].These variations can be attributed to the E. tarda strain, the fish's weight and size, and accommodation conditions.This information is crucial for understanding the impact of E. tarda on different fish species.
We found that E. tarda was sensitive to Trimethoprim & Sulfamethoxazole, showed intermediate sensitivity to florfenicol, and was resistant to several other antibiotics tetracycline, ciprofloxacin, erythromycin, gentamycin, amoxicillin, ampicillin kanamycin, cefotaxime, and streptomycin.However, Kumar et al. [63] reported that E. tarda strains were multiply resistant to antibiotics, with a high percentage of isolates retrieved from aquatic animals in West Bengal and Bihar, India.These findings underscore the urgent need for further research into alternative treatment strategies, highlighting the importance of our collective efforts in this field.
In this experiment, the growth performance was enhanced in Nile tilapia (45.05 ± 0.4 g), fed S. platensis at doses of 5 and 10 g/kg fish feed.Similar findings with different fish species were recorded by Adel et al. [64], who stated that growth performance and feed utilization of great sturgeon (Huso huso) were improved with S. platensis supplementation at a dose of 100 g/kg fish feed.Also, Mabrouk et al. [65] had similar findings with Nile tilapia fed on dietary S. platensis at 10 /kg fish feed.Some authors fed fish on S. platensis meal and had similar outcomes.Also, Rahman et al. [66] found that Stinging catfish (Heteropneustes fossilis) fed a diet containing S. platensis meal (50 g/kg fish feed) obtained significantly improved WG and DWG than the control ones.Furthermore, Nile tilapia fed dietary S. platensis could utilize feed ingredients more efficiently, so it could be considered an essential element of Nile tilapia natural diet [67].
The importance of natural barriers, including inflammatory cytokine, in the fight against bacterial infections is well known.The pro-inflammatory cytokines (IL-1β and TNF-α) are the supporting columns of humoral cell-mediated immune responses, and they activate macrophages, production of (nitric oxide reactive oxygen species, and reactive nitrogen intermediates), and autophagy [68].In this work, gene expression of IL-1β and TNF-α were upsurged in response to E. tarda infection by 3 h, with more intensity in fish that received dietary S. platensis.Furthermore, the expression of IL-1β and TNF-α was subsided with lower intensity after 48 h post-infection.This finding was consistent with Mayer-Barber and Yan [69], who claimed that IL-1β is a rapid cytokine that responds to acute bacterial infections; it also stimulates the secretion of chemokines that are required for the optimal control neutrophil-dependent. Likely, the immunity of Nile tilapia was significantly enhanced by feeding S. platensis-diets at 1% more than 2% with a rapid down-regulation of anti-inflammatory cytokine (IL-10) [70].Also, Awad et al. [71] found that the serum of Nile tilapia received dietary S. platensis had a low content of TNF-α and IL-10, whereas after A. hydrophila infection, a significant rise in TNF-α and a significant downregulation in IL-10 in control.This finding was inconsistent with our study as the infected fish (control) was immunosuppressed, and the inflammatory status persisted.Controversially, Song et al. [72] claimed that grass carp (Ctenopharyngodon idella) infected with  A. hydrophila, intestinal pro-inflammatory cytokines (IL-1, IL-8, and TNF-α) were upregulated, and dietary S. platensis significantly reduced TNF-α and elevates IL-10.This difference was due to sampling time, as our samples were collected directly after 3 and 48 h post-infection.In this experiment, MR% of Nile tilapia challenged with E. tarda was decreased, especially those who received 10 g S. platensis /kg fish feed compared with those of the control and ones who received 5 g S. platensis /kg fish feed, 46.67%, 60%, 60%, respectively.A 10 g S. platensis / kg fish feed dose could provide a 22.2% RPL.Consistent with these findings, dietary S. platensis had a potential immune role in different fish species, enhancing survivability and boosting the resistance against fish pathogens [64].Similarly, African catfish and Nile tilapia injected with LD 50 (0.2 ml of 10 4 CFU/ml) at 25 °C resulted in MR of 70% and 60% [62].Consistently, dietary S. platensis increased the resistance against A. hydrophila infection in African Catfish [73], Cyprinus Carpio [26], and Gibel carp (Carassius gibelio) [74].These findings could be due to the high immune response in Nile tilapia received S. platensis compared to the control.Similarly, common carp (Cyprinus carpio) fed dietary S. platensis showed high cytokines generation with rapid onset [26].Moreover, a small dose of S. platensis (10 mg/fish /day) could improve the general health of Nile tilapia.Also, it could stimulate the immune system, making fish more resistant to infectious diseases [75].Also, Tawfeek et al. [76] claimed that dietary Chlorella vulgaris algae could mitigate chlorpyrifos toxicity in Nile tilapia, protecting against Streptococcus agalactiae infection.
Nile tilapia experimentally infected E. tarda in this work, drastically altering their behavior: high OM, low food apprehension, and abnormal swimming.Also, it was noticed that dietary S. platensis could ameliorate these withdrawals with a fast restoration of normal status.Inconsistency acute and chronic stress in aquatic animals is usually accompanied by changes in feeding behavior, respiratory activity, escaping behavior, swimming style, and abnormal behavior [77].Similarly, Nile tilapia experimentally infected E. tarda with lethargy and showed abnormal swimming behavior and loss of escape reflexes [61].These changes in fish behavior could be due to insufficient oxygen supply to tissues, increasing compensatory respiration in response to stress and anemia [78,79].
Histopathological findings revealed gills truncated lamellae and tips sloughing lamellar aneurysm in control fish infected with E. tarda.Hepatic tissue of control and S. platensis groups showed normal.In contrast, hepatic parenchyma necrosis in the form of a wide area of lysed hepatic-parenchyma, fish received S. platensis and infected with E. tarda.The spleen of fish infected with E. tarda showed marked depletion of lymphocytic elements with the absence of melano-macrophage centers.Muscles showed foci of bacteria aggregation and vacuolation.Similarly, E. ictaluri infected farmed hybrid red tilapia (Oreochromis sp.) in Southeast Asia, muscles had various clumps of rod-shaped bacteria stained with hematoxylin were also visualized; the presence of cell pyknosis and karyorrhexis was commonly found in the infected spleen with increasing numbers of melanoma-macrophage centers (MMCs).Also, they added that severe blood congestion, hemorrhage, and necrotic hepatocytes were observed in the hepatic tissue [80].In accordance, Pirarat et al. [81] stated that lymphoid depletion and lymphoid necrosis were noticeable in control Nile tilapia that was infected with E. tarda, while focal secondary lamellar fusions and severe anemia were observed only in the gills; also they added that L. rhamnosus (probiiotic) could mitigate impacts of experimental E. tarda infection.These pathological lesions resulted from toxins produced by E. tarda while antioxidant contents of S. platensis could ameliorate such damages.Accordingly, Pseudomonas fluorescens caused oxidative stress in Nile tilapia, causing severe tissue damage, and fish fed on S. platensis (1%) Fig. 8 Liver with evidence of liquefactive necrosis in the form of a wide area of lysed hepatic-parenchyma (d), normal hepatic parenchyma, development of pancreatic acini (a, b &c) with occurrence of some melano-macrophage cells (a&b).Small lysed area still detected in hepatic parenchyma (e) Note (a); control fish, (b); fish received 5 g/kg fish feed S. platensis, (c); fish received 10 g/kg fish feed S. platensis, (d); control infect with E. tarda, (e); fish received S. platensis and infect with E. tarda.H&E X 400 showed hyperplasia of melanoma-macrophage centers and hemopoietic elements S. platensis (2%) also showed hyperplasia of melanoma-macrophage center and more activation of hemopoietic tissue [70].

Conclusion
Seventeen Nile tilapia out of 50 fish were infected with E. tarda, which harbored clinical and post-mortem signs resembling experimental fish.The level of NH 3 was 0.11-0.15mg/Lin fish farm, which was stressful for Nile tilapia.The bacterial strain was identified using API20 E (4,144,000) and molecular methods E. tarda strain and deposited in GenBank (PP449014).E. tarda (JALO4) was positive for gyrB1 and edw1 AHL-synthase, while LD 50 was determined to be 1.7 × 10 5 CFU/mL.E. tarda was sensitive to trimethoprim & sulfamethoxazole, with an MDR of 0.8, indicating a multiple antibiotic-resistant bacteria.Higher operculum movement, low food apprehension, and abnormal swimming were observed in experimentally infected fish.The expression IL-1β and TNF-α were intensively upregulated in E. tarda-challenged fish in S. platensis groups.Dietary S. platensis at a dose of 10 g/kg fish feed could provide a higher RPL of 22.2% Nile tilapia challenged against E. tarda.

Fig. 4 Fig. 3
Fig. 4 Gene expression of IL-1β in the head kidney of Nile tilapia experimental infected with E. tarda.Different letters indicate significant difference at P ≤ 0.05.(-); negative control fish were injected with saline, (+); positive control fish were injected with E. tarda

Fig. 5
Fig. 5 Gene expression of TNF-α in the head kidney of Nile tilapia experimental infected with E. tarda.Different letters indicate significant difference at P ≤ 0.05.(-); negative control fish were injected with saline, (+); positive control fish were injected with E. tarda

Table 1
Primers sequences, target genes, amplicon sizes and cycling conditions

Table 2
Data on bacterial strains, accession number, site, virulence genes, and multidrug resistant genes Note +/-; positive/negative

Table 3
Feeding trial and growth performance of the experimental Nile Tilapia.(mean ± standard error)

Table 4
Mortality rate of Nile tilapia experimentally infected with E. tarda JALO4 and S. platensis treatment

Table 5
Fish behavioral reflexes at 3 and 14 days post-infection Note (-); negative control fish were injected with saline, (+); positive control fish were injected with E. tarda